Casa
scp 7143 j

Transgenic Mice Expressing Green Fluorescent Protein under the

Por um escritor misterioso

Descrição
The melanocortin-4 receptor (MC4-R) is an important regulator of energy homeostasis, and evidence suggests that MC4-R-expressing neurons are downstream targets of leptin action. MC4-Rs are broadly expressed in the CNS, and the distribution of MC4-R mRNA has been analyzed most extensively in the rat. However, relatively little is known concerning chemical profiles of MC4-R-expressing neurons. The extent to which central melanocortins act presynaptically or postsynaptically on MC4-Rs is also unknown. To address these issues, we have generated a transgenic mouse line expressing green fluorescent protein (GFP) under the control of the MC4-R promoter, using a modified bacterial artificial chromosome. We have confirmed that the CNS distribution of GFP-producing cells is identical to that of MC4-R mRNA in wild-type mice and that nearly all GFP-producing cells coexpress MC4-R mRNA. For example, cells coexpressing GFP and MC4-R mRNA were distributed in the paraventricular hypothalamic nucleus (PVH) and the dorsal motor nucleus of the vagus (DMV). MC4-R promotor-driven GFP expression was found in PVH cells producing thyrotropin-releasing hormone and in cholinergic DMV cells. Finally, we have observed that a synthetic MC3/4-R agonist, MT-II, depolarizes some GFP-expressing cells, suggesting that MC4-Rs function postsynaptically in some instances and may function presynaptically in others. These studies extend our knowledge of the distribution and function of the MC4-R. The transgenic mouse line should be useful for future studies on the role of melanocortin signaling in regulating feeding behavior and autonomic homeostasis.
Transgenic Mice Expressing Green Fluorescent Protein under the
Kaede mice for in vivo monitoring of cell migration
Transgenic Mice Expressing Green Fluorescent Protein under the
Green fluorescent protein (GFP) in plant research
Transgenic Mice Expressing Green Fluorescent Protein under the
Generation of transgenic cynomolgus monkeys that express green
Transgenic Mice Expressing Green Fluorescent Protein under the
Biotech's Glowing Breakthrough
Transgenic Mice Expressing Green Fluorescent Protein under the
A Nanobody-Based System Using Fluorescent Proteins as Scaffolds
Transgenic Mice Expressing Green Fluorescent Protein under the
IJMS, Free Full-Text
Transgenic Mice Expressing Green Fluorescent Protein under the
Germline mutagenesis mediated by Sleeping Beautytransposon system
Transgenic Mice Expressing Green Fluorescent Protein under the
Protein Delivery: If Your GFP (or Other Small Protein) Is in the
Transgenic Mice Expressing Green Fluorescent Protein under the
A Transgenic Mouse Line Expressing the Red Fluorescent Protein
Transgenic Mice Expressing Green Fluorescent Protein under the
Frontiers Fluorescent transgenic mouse models for whole-brain
Transgenic Mice Expressing Green Fluorescent Protein under the
004353 - UBI-GFP Strain Details
Transgenic Mice Expressing Green Fluorescent Protein under the
Green Fluorescent Protein in Transgenic Mice
Transgenic Mice Expressing Green Fluorescent Protein under the
A Transgenic Mouse Line Expressing the Red Fluorescent Protein
Transgenic Mice Expressing Green Fluorescent Protein under the
Introducing AntiCancer
de por adulto (o preço varia de acordo com o tamanho do grupo)